Inflammatory markers reduced by single 80,000 IU of vitamin D (Saudi) – Sept 2022

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A Single Vitamin D3 Bolus Supplementation Improves Vitamin D Status and Reduces Proinflammatory Cytokines in Healthy Females

Nutrients 2022, 14(19), 3963; https://doi.org/10.3390/nu14193963
by Hadeil M. Alsufiani, et. al

Vitamin D deficiency is a global health problem that not only leads to metabolic bone disease but also to many other illnesses, most of which are associated with chronic inflammation. Thus, our aim was to investigate the safety and effectiveness of a single high dose of vitamin D3 (80,000 IU) on vitamin D status and proinflammatory cytokines such as interleukin (IL)6, IL8 and tumor necrosis factor (TNF) in healthy Saudi females. Fifty healthy females were recruited and orally supplemented with a single vitamin D3 bolus (80,000 IU). All participants donated fasting blood samples at baseline, one day and thirty days after supplementation. Serum 25-hydroxyvitamin D3 (25(OH)D3), IL6, IL8, TNF, calcium, phosphate, parathyroid hormone (PTH) and blood lipid levels were determined. Serum 25(OH)D3 significantly increased one and thirty days after supplementation when compared with baseline without causing elevation in calcium or phosphate or a decrease in PTH to abnormal levels. In contrast, the concentrations of the three representative proinflammatory cytokines decreased gradually until the end of the study period.
In conclusion, a single high dose (80,000 IU) is effective in improving serum vitamin D status and reducing the concentration of the proinflammatory cytokines in a rapid and safe way in healthy females.
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Similar study published 1 month later for males in a different journal

A Single Oral Vitamin D3 Bolus Reduces Inflammatory Markers in Healthy Saudi Males
Int J Mol Sci. 2022 Oct 9;23(19):11992. doi: 10.3390/ijms231911992.
Shareefa A AlGhamdi 1 2 3 , Nusaibah N Enaibsi 1 , Hadeil M Alsufiani 1 2 3 , Huda F Alshaibi 1 3 , Sawsan O Khoja 1 2 , Carsten Carlberg 4 5
1Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia
2 Vitamin D Pharmacogenomics Research Group, King Abdulaziz University, Jeddah 21589, Saudi Arabia
3 Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University,
Jeddah 21589, Saudi Arabia
4 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland
5 Institute of Biomedicine, School of Medicine, University of Eastern Finland, 70211 Kuopio, Finland
* Correspondence: saaalghamdi1 at kau.edu.sa (S.A.A.); c.carlberg at pan.olsztyn.pl (C.C.)

Vitamin D deficiency has increased in the general population and is a public health issue. Vitamin D plays an important role in regulating the immune system, e.g., by modulating the production of inflammatory cytokines. In most countries, the recommended maximal daily dose of vitamin D3 is 4000 IU (100 µg) per day. In this study, we investigated whether a single vitamin D3 bolus can reduce the levels of the inflammatory markers interleukin (IL) 6, IL8 and tumor necrosis factor (TNF) within one month. Fifty healthy Saudi males were recruited from the local community in Jeddah city and were orally supplemented with a single dose of 80,000 IU vitamin D3. Serum samples were collected at time points 0, 1 and 30 days, and serum levels of IL6, IL8 and TNF, parathyroid hormone (PTH), 25-hydroxyvitamin D3 (25(OH)D3), triglycerides, cholesterol, calcium (Ca2+) and phosphate (PO4-) were determined. On average, the vitamin D3 bolus resulted in a significant increase in vitamin D status as well as in a significant decrease in the levels of inflammatory cytokines even one month after supplementation without changing serum Ca2+, PO4- or lipid levels. In conclusion, single high-dose vitamin D3 supplementation is safe for reducing inflammation markers and may lead to an update of current recommendations for vitamin D intake, in order to prevent critical health problems.
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VitaminDWiki - 3 studies in both categories Inflammation and Loading Dose

This list is automatically updated

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VitaminDWiki - Loading Dose of Vitamin D category contains

206 items in category
see also Overview Loading of vitamin D   Overview Toxicity of vitamin D
Better than Daily 1: Fewer chances to forget, 2) Gets past receptor barrier
Injection category has 64 items
 
It appears that over 1 million Vitamin D loading doses have been taken
Doses ranged from 100,000 to 600,000 IU over a period of a day to a month
No reports of serious adverse reactions
Many studies report on the benefits resulting from loading doses

TOP articles in Loading Dose of Vitamin D

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VitaminDWiki - Inflammation category contains

173 items total

Example studies

• Vitamin D energizes the innate and adaptive immune systems to fight lung inflammation – Sept 2022
• COVID-19 inflammation extinguished by 60,000 IU of vitamin D nanoemulsion daily for a week – RCT May 2021
• Inflammation (C-reactive protein) associated with low vitamin D in 22 diseases – April 2020
• Vitamin D reduces inflammation in critically ill patients – Sept 2020
• Inflammation is reduced by each of the following: Vitamin D, Omega-3, Diet
• How Vitamin D reduces inflammation, improves immunity and fights autoimmunity – review Dec 2018
• CYTOKINE in VitaminDWiki 12 titles as of July 2023
• 17 pages in VitaminDWiki had REACTIVE or CRP in title as of July 2023
• Little risk of infection after surgery if have more than 50 ng of vitamin D - 2014

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VitaminDWiki - Better than Daily contains

33 items in BETTER THAN DAILY category

Non-daily (Bolus) is better:

1. Better compliance for everyone
   • Fewer opportunities to forget.
   • If happen to forget, just take the dose many days later
   • Fewer times to have to take a pill - for those who dislike doing so
2. Non-daily gets more vitamin D to the cells for the ~20% who have a poor Vitamin D Receptor
   • A high concentration gradient is one of 14+ ways to get past Vitamin D Receptor limitations
   • So, while 80% get no extra benefit from non-daily dosing, 20% will get an extra benefit

• Weekly Vitamin D better than daily – lower cost, less pill burden, perhaps 1.5X better response – meta-analysis Oct 2024
• Vitamin D given daily, weekly, or monthly has similar response (116 RCTs) – meta-analysis Aug 2023
• 44 percent of successful RCTs in VitaminDWiki used non-daily dosing - Nov 2020
• Monthly vitamin D dosing better for children than daily (again) - Oct 2023

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VitaminDWiki - 50,000 IU of Vitamin D once every two weeks proven to prevent/treat:

Diabetes + Heart Failure + Chronic Pain + Depression + Autism + Breast Cancer + Colon Cancer + Prostate Cancer + BPH (prostate) + Preeclampsia + Premature Birth + Falls + Cognitive Decline + Respiratory Tract Infection + Influenza + Tuberculosis + Chronic Obstructive Pulmonary Disease + Lupus + Inflammatory Bowel Syndrome + Urinary Tract Infection + Poor Sleep + Growing Pain + Multiple Sclerosis + PMS + Schizophrenia + Endometriosis + Smoking    27 problems
   Note: Once a week also fights: COVID, Headaches, Colds, Fibromyalgia, Asthma, Hives, Colitis etc.

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Response to a single dose of 100,000 IU